rabbit polyclonal anti interferon γ  (Bioss)

Journal: Veterinary Immunology and Immunopathology

Article Title: Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease

doi: 10.1016/j.vetimm.2017.10.001

Figure Lengend Snippet: Intracellular staining of IFN-γ in transfected CHO cells. A) CHO cells were either transfected with plasmids containing the chicken IFN-γ gene or empty plasmids (mock controls). Cells (day 22 after transfection) were cultured with or without BFA for 18 hours and subsequently stained intracellularly using monoclonal anti-chicken IFN-γ antibody (Mab80) conjugated with APC. Representative samples are show with frequency of IFN-γ+ cells indicated above gate. B) Comparison of transfected CHO cells (day 30 after transfection) cultured with 10 μg/ml BFA for 18 hours and subsequently stained intracellularly with either commercial ELISA capture antibody (5C.123.08) & A647 conjugated secondary anti-mouse IgG1 or monoclonal anti-chicken IFN-γ antibody directly conjugated with APC (Mab80) or commercial ELISA detection antibody (5C.123.02) directly conjugated with APC or polyclonal rabbit anti-chicken IFN-γ antibody & FITC-conjugated secondary goat anti-rabbit IgG. Representative samples are show with frequency of IFN-γ+ cells indicated above gate.

Article Snippet: Subsequently, the cells were washed twice with BD Perm/Wash buffer for 5 min at 600 × g , followed by staining with rabbit polyclonal anti-chIFN-γ IgG antibody (BioRad) and secondary goat anti-rabbit IgG FITC (Beckman Coulter, Brea, CA, cat no 732745).

Techniques: Staining, Transfection, Cell Culture, Comparison, Enzyme-linked Immunosorbent Assay

Journal: Veterinary Immunology and Immunopathology

Article Title: Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease

doi: 10.1016/j.vetimm.2017.10.001

Figure Lengend Snippet: Intracellular staining of IFN-γ in transfected CHO cells. A) CHO cells were either transfected with plasmids containing the chicken IFN-γ gene or empty plasmids (mock controls). Cells (day 22 after transfection) were cultured with or without BFA for 18 hours and subsequently stained intracellularly using monoclonal anti-chicken IFN-γ antibody (Mab80) conjugated with APC. Representative samples are show with frequency of IFN-γ+ cells indicated above gate. B) Comparison of transfected CHO cells (day 30 after transfection) cultured with 10 μg/ml BFA for 18 hours and subsequently stained intracellularly with either commercial ELISA capture antibody (5C.123.08) & A647 conjugated secondary anti-mouse IgG1 or monoclonal anti-chicken IFN-γ antibody directly conjugated with APC (Mab80) or commercial ELISA detection antibody (5C.123.02) directly conjugated with APC or polyclonal rabbit anti-chicken IFN-γ antibody & FITC-conjugated secondary goat anti-rabbit IgG. Representative samples are show with frequency of IFN-γ+ cells indicated above gate.

Article Snippet: All monoclonal antibodies for phenotypic markers were obtained from Southern Biotech (Birmingham, AL, USA) except the rabbit polyclonal anti-chIFN-γ antibody, which was purchased unlabelled and FITC conjugated using the LYNX Rapid Fluorescein Conjugation Kit ® (BioRad) following the manufacturer’s instructions.

Techniques: Staining, Transfection, Cell Culture, Comparison, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Decreased PPAR-γ expression after internal carotid artery stenting is associated with vascular lesions induced by smooth muscle cell proliferation and systemic inflammation in a minipig model

doi:

Figure Lengend Snippet: Immunohistochemical staining for peroxisome proliferator-activated receptor gamma (PPAR-γ) expression in the vascular smooth muscle cells (VSMCs) in each group. The immunohistochemical staining was conducted 90 days after stenting. A: Normal control group, B: Stenting group, C: Stenting plus rosiglitazone (ROSI) treatment group. PPAR-γ-positive VSMCs are shown by their nuclear brown staining (×100).

Article Snippet: After deparaffinization and hydration, the specimens were blocked with 0.3% H 2 O 2 at room temperature for 15 min. A rabbit polyclonal antibody against pig-PPAR-γ (2.5 μg/ml, ABD Serotec, USA) was used for immunohistochemical staining.

Techniques: Immunohistochemical staining, Staining, Expressing, Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Decreased PPAR-γ expression after internal carotid artery stenting is associated with vascular lesions induced by smooth muscle cell proliferation and systemic inflammation in a minipig model

doi:

Figure Lengend Snippet: Western blot assay for the expression of minipig carotid artery peroxisome proliferator-activated receptor gamma (PPAR-γ) and smooth muscle 22-alpha (SM22α). The carotid artery tissues were harvested and the total protein was extracted for the western blot analysis by using anti-PPAR-γ, anti-SM22α, and anti-β-actin primary antibodies. The immunocomplexes were detected using horseradish peroxidase-conjugated secondary antibodies, followed by enhanced chemiluminescence. The relative molecular masses of the target proteins are indicated.

Article Snippet: After deparaffinization and hydration, the specimens were blocked with 0.3% H 2 O 2 at room temperature for 15 min. A rabbit polyclonal antibody against pig-PPAR-γ (2.5 μg/ml, ABD Serotec, USA) was used for immunohistochemical staining.

Techniques: Western Blot, Expressing